Table 2. Quantitative Analysis of Immunoreactive Neutrophils and Immunostaining in the Lung Sections
*P < 0.0001 comparing control and lipopolysaccharide.
†P < 0.0001 comparing lipopolysaccharide and cyclophosphamide + lipopolysaccharide.
‡P < 0.0001 comparing lipopolysaccharide and lipopolysaccharide + nitric oxide (NO).
Total = neutrophil number in alveoli in a 50-μm2area; Positive = immunopositive neutrophil number in the alveoli in a 50-μm2area; Mean Density = mean density of red color image of each RGB image of 8-bit resolution in each color; Control = aerosolized with saline and placed in room air for 6 h; Lipopolysaccharide = exposed (aerosolized) to lipopolysaccharide and placed in room air for 6 h; Cyclophosphamide + lipopolysaccharide = after intraperitoneal injection of cyclophosphamide aerosolized with lipopolysaccharide and placed in room air for 6 h; Lipopolysaccharide + NO = exposed to lipopolysaccharide and placed in 20 ppm nitric oxide for 6 h.