Fig. 4. Effect of PD98059 on the midazolam-induced vascular endothelial growth factor (VEGF) release from A10 cells. Cultured cells were pretreated with various concentrations of PD98059 for 60 min, then stimulated by 0.1 mm midazolam (•) or treated with vehicle (○) (each for 60 h). Each value represents the mean ± SD of triplicate determinations. Similar results were obtained in two other cell preparations. *P is less than 0.05 compared with midazolam alone.