Fig. 5. Effect of U0126 on the midazolam-induced vascular endothelial growth factor (VEGF) release from A10 cells. Cultured cells were pretreated with various concentrations of U0126 for 60 min, then stimulated by 0.1 mm midazolam or treated with vehicle (each for 60 h). Each value represents the mean ± SD of triplicate determinations. Similar results were obtained in two other cell preparations. *P  is less than 0.05 compared with midazolam alone.