![Fig. 4. The effects of 10 μm atropine (n = 4), 100 μm acetylcholine (n = 4), and 1 mm halothane (n = 6) on the amount of [35S]GTPγS bound by endogenous Gαiimmunoprecipitated from membrane preparation, assayed at 10 min. The amount of bound [35S]GTPγS, expressed as femtomoles [35S]GTPγS bound per milligrams total protein, are the mean of the specified number of measurements, done in duplicate, and with a paired control. None of the three significantly affected the [35S]GTPγS binding as judged by one-way repeated-measures analysis of variance. GTP = guanosine 5′-triphosphate.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/99/1/10.1097_00000542-200307000-00019/2/19ff4.png?Expires=1717029917&Signature=nw1lJ5s5LAl-gIVzC3K1BmLJPhvVjvvsucWcGz1GBY9QfxPsj5MdA3t3MhmIxY8pRZ36xbA1zSDVxSFAKMfNoL4L5dSlZavQcfYcFSuK8KL9-7L7lLWNRiyxXTh1TfIX4SoIAieCGhSq09aZFzSTGS6LEV4zpODyGb9P19UQR5ZCE~zvuLLTIr2qfeU8dVxDIb-W0lDbVTGOK~vbjQUJ6B8s1WESe9Wdde4c6XET4piOSKUdSuLeEMqbGOp6GjN5anwPU36rJlgN3VNiAIC1uc74sbtZliorq4v4RTSjBRouEAxjoQCgQgBzk8pWvISWW5mVSnz7djZyYmD~TmVG~w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 4. The effects of 10 μm atropine (n = 4), 100 μm acetylcholine (n = 4), and 1 mm halothane (n = 6) on the amount of [35S]GTPγS bound by endogenous Gαiimmunoprecipitated from membrane preparation, assayed at 10 min. The amount of bound [35S]GTPγS, expressed as femtomoles [35S]GTPγS bound per milligrams total protein, are the mean of the specified number of measurements, done in duplicate, and with a paired control. None of the three significantly affected the [35S]GTPγS binding as judged by one-way repeated-measures analysis of variance. GTP = guanosine 5′-triphosphate.
