Fig. 4. Protein kinase C ε (PKC ε) colocalization with subcellular targets in response to ischemic preconditioning (IPC ) and anesthetic preconditioning (APC ). (A and B ) There was clear translocation of PKCε (red ) to sarcolemma (green ) in IPC and APC (colocalization indicated in yellow ), which was inhibited by chelerythrine (CHE ) (IPC, 10 μm; APC, 5 μm). (C ) No translocation of PKCε (red ) to the mitochondria (green ) was observed in both types of preconditioning. There was constitutively translocated PKCε to the intercalated disks in control hearts (CTLLD= hearts with time-matched perfusion on the Langendorff apparatus and hearts directly taken from the animals without prior perfusion [CTLAN]). Pictures represent epifluorescence micrographs with ×400 magnification. *Significantly increased compared with CTLLDor CTLAN, P < 0.05. †Not significantly different from CTLLDor CTLAN.