Fig. 2. Coapplication of low concentrations of solanaceous glycoalkaloids (SGAs) with either neostigmine or pancuronium results in additive inhibition of butyrylcholinesterase (BuChE) activity. (A  and B ) Effect of neostigmine on BuChE activity in the absence (filled symbols) and presence of either 100 nm α-solanine (A , open symbols) or 30 nm α-chaconine (B , open symbols). (C  and D ) Effect of pancuronium on BuChE activity in the absence (filled symbols) and presence of either 100 nm α-solanine (C , open symbols) or 30 nm α-chaconine (D , open symbols). Data points are mean ± SEM of at least three experiments, with each determination carried out in triplicate. Arrows denote the range of clinically relevant serum concentrations (see table 2for references). Error bars not visible are within symbol size.