Fig. 3. (  A ) Monocyte chemoattractant protein 1 (MCP-1) protein determination in bronchoalveolar lavage fluid (BALF). Animals were pretreated with control liposomes (co lip) or clodronate liposomes (clodr.). After 72 h, phosphate-buffered saline (PBS;  white bars ) or 0.1N acidic solution (HCl;  black bars ), pH 1, was instilled intratracheally, and the lungs were analyzed 6 h later. Lungs were lavaged, and MCP-1 was determined using a standard enzyme-linked immunosorbent assay protocol. Values are presented as mean ± SEM from five animals. (  B ) Macrophage inflammatory protein 1β (MIP-1β) protein determination in BALF. Animals were pretreated with control liposomes or clodronate liposomes. After 72 h, PBS (  white bars ) or HCl (  black bars ), pH 1, was instilled intratracheally, and the lungs were analyzed 6 h later. Lungs were lavaged, and MIP-1β was determined by Western blotting. Values are presented as mean ± SEM from five animals. (  C ) Cytokine-induced neutrophil chemoattractant 1 (CINC-1) protein determination in BALF. Animals were pretreated with control liposomes or clodronate liposomes. After 72 h, PBS (  white bars ) or HCl (  black bars ), pH 1, was instilled intratracheally, and the lungs were analyzed 6 hlater. Lungs were lavaged, and CINC-1 was determined using a standard enzyme-linked immunosorbent assay protocol. Values are presented as mean ± SEM from five animals. (  D ) Macrophage inflammatory protein 2 (MIP-2) protein determination in BALF. Animals were pretreated with control liposomes or clodronate liposomes. After 72 h, PBS (  white bars ) or HCl (  black bars ), pH 1, was instilled intratracheally, and the lungs were analyzed 6 h later. Lungs were lavaged, and MIP-2 was determined using a standard enzyme-linked immunosorbent assay protocol. Values are presented as mean ± SEM from five animals.