Fig. 8. (  A ) Enzyme-linked immunosorbent assay for albumin content in bronchoalveolar lavage fluid (BALF). Phosphate buffered-saline (PBS;  white bars ) or 0.1N acidic solution (HCl;  black bars ), pH 1, was instilled intratracheally. Three hours after the onset of lung injury, pyrrolidine dithiocarbamate (PDTC) or PBS as a control was intratracheally instilled, and the lungs were analyzed 6 h after the onset of injury. Values are presented as mean ± SEM from five animals. (  B ) Hemoglobin (Hb) content in BALF. PBS (  white bars ) or HCl ( black bars ), pH 1, was instilled intratracheally. Three hours after the onset of lung injury, PDTC or PBS as a control was intratracheally instilled, and the lungs were analyzed 6 h after the onset of injury. Erythrocytes were lysed and hemoglobin was determined by spectrophotometer at 540 nm. One PBS + PBS value was defined as 1; all others were adapted. Values are presented as mean ± SEM from five animals.