![Fig. 4. (A–E ) Representative traces of [Ca2+]cyt-changes with sequential stimulation using 100 mm KCl followed by 1 mm carbachol. (A ) Control. (B–F ) Cells were pretreated for 5 min with 10 mm caffeine (B ), 1 μm thapsigargin (C ), 10 mm caffeine and 1 μm thapsigargin (D ), 10 mm caffeine and 1 mm carbachol (E ), or with 1 μm thapsigargin and 1 mm carbachol (F ). (G, H ) Statistical summary. Values presented are mean ± SEM (n = 2–19). The significance of the differences among different groups was tested using the Student-Neuman-Keuls test and labeled as follows:*P < 0.05 to the control group; $ P < 0.05 to the thapsigargin group.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/92/6/10.1097_00000542-200006000-00035/2/35ff4.png?Expires=1716445557&Signature=GLr87kRMGLDF4AmscGOgBMNnSQTboSSY7abQ7bswiOrD2AY8-lgtlkMVA6wdkFHWHr30DlhnDzqeMqe31Hiffp9Eqgha0bDlx-FDjk~5lGIrqP-qVpp0jfZz~BEioBjiQyA-G042galiWBp9~D8bcxNxHh1hpNhYx06uVYh4boxsP-FsPx3gdly7qzuJsxwylUKKDlWiBNF2HpBaSlRMoZLHyqalkR9u~i6UWCV~sdU47JQGuQEVCwyWNji8Gn60UkbwYdJEncl-hzZjlJxG91Zy8tBvoy8GZlmx1UyTryx4~3YOlpQwzRrNS8QxldHXwrowFCkFQg5ROXEkG7RBMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 4. (A–E ) Representative traces of [Ca2+]cyt-changes with sequential stimulation using 100 mm KCl followed by 1 mm carbachol. (A ) Control. (B–F ) Cells were pretreated for 5 min with 10 mm caffeine (B ), 1 μm thapsigargin (C ), 10 mm caffeine and 1 μm thapsigargin (D ), 10 mm caffeine and 1 mm carbachol (E ), or with 1 μm thapsigargin and 1 mm carbachol (F ). (G, H ) Statistical summary. Values presented are mean ± SEM (n = 2–19). The significance of the differences among different groups was tested using the Student-Neuman-Keuls test and labeled as follows:*P < 0.05 to the control group; $ P < 0.05 to the thapsigargin group.
