Fig. 1. Baseline responses of acid-sensing ion channel 2 (ASIC2) knockout (KO) and wild-type (WT) mice did not differ. Data are shown as mean ± SE in all figures. (A ) ASIC2 KO (n = 20) and WT (n = 24) mice showed a similar stimulus response function to application of a punctate mechanical stimulus. The average percentage of trials that elicited a withdrawal response after application of a von Frey probe is plotted as a function of force. (B ) ASIC2 KO mice and WT mice showed a similar response to stroking of the foot with a von Frey filament. The average percentage of trials in which the animals responded to the stroking stimulus is plotted. Values in parentheses indicate sample sizes. (C ) ASIC2 KO mice and WT mice showed a similar response to radiant heat stimuli. The average withdrawal latency is plotted. For a given mouse, the baseline responses obtained after stimulation of the left and right paw were averaged together. Values in parentheses indicate sample sizes. ***P < 0.0001 main effect of sex. F = female; M = male.