Fig. 9. Representative lung sections of mice subjected to high tidal volume (HVT) ventilation in the presence and absence of 60 ppm hydrogen sulfide (H2S) or after intravascular administration of sodium sulfide (Na2S). These sections were stained with hematoxylin and eosin (H/E) or reacted with antibodies (AB) against neutrophils (inset : higher magnification) and were compared with sections from nonventilated control mice. In control mice, the airway epithelium (open arrows ) is intact, no edema surrounding the airway is seen, and few neutrophils are visible (thin black arrows ). HVTventilation induces airway disruption, edema formation between airways and adjacent vessels (thick black arrows ), and neutrophil infiltration. Similar pathologic changes were present in lungs subjected to HVTventilation in the presence of 60 ppm H2S. Less epithelial disruption and edema and fewer neutrophils are seen in lung tissue sections after Na2S pretreatment.