Fig. 8. Alterations occurred in inhibitory synaptic transmission in pyramidal cells of subiculi after exposure to anesthesia early in life. (A ) Representative evoked inhibitory postsynaptic currents (eIPSCs) obtained using a paired-pulse protocol to record from two pyramidal cells in the subiculi of rats in the control (red line ) and experimental groups (blue line ). Note that the experimental group had decreased current amplitude and faster decay. Arrows  indicate the time of paired-pulse stimulus application (P1 = stimulus 1 and P2 = stimulus 2; interval 1.1 s). Stimulus transients have been removed for clarity of the current traces. (B ) Histogram showing average data from control cells (n = 14) and experimental cells (n = 12). Red bars  indicate control cells; blue bars  represent experimental cells; vertical lines  indicate the SEM of multiple determinations. All data are normalized to 100% of average responses in the control group. Left bars show a decrease in net charge transfer of eIPSCs from 100 ± 18% to 51 ± 9% (*P < 0.05) in the experimental group; middle bars show a decrease in decay of τ from 100 ± 16% to 59 ± 7% (*P < 0.05); right bars show a small but significant increase in P2/P1 from 100 ± 2% to 107 ± 1% (*P < 0.05).