Fig. 3.
Molecular second-generation (F1) effects of young adult parental exposure to sevoflurane. (A–H) The respective levels of sodium ion-potassium ion-chloride ion cotransporter NKCC1 gene (Nkcc1) mRNA and potassium ion-chloride ion cotransporter KCC2 gene (Kcc2) mRNA in the hypothalamus of F1 males (A and B) and F1 females (C and D) and in the hippocampus of F1 males (E and F) and F1 females (G and H). F1 rats were categorized as the offspring of (1) control males plus control females (con-M*con-F); (2) exposed males plus control females (sevo-M*con-F); (3) control males plus exposed females (con-M*sevo-F); and (4) exposed males plus exposed females (sevo-M*sevo-F). Data normalized against control are means ± SD from 6 rats per group (n = 5, female hypothalamic Nkcc1 in con-M*con-F). *P < 0.05 versus con-M*con-F. (I–L) The levels of corticotropin-releasing hormone gene (Crh) mRNA in the hypothalamus and glucocorticoid receptor gene (Gr) mRNA in the hippocampus in F1 males (I and J) and F1 females (K and L). Data normalized against control are means ± SD from 5 or 6 rats per group. Multiple pairwise comparisons were done with the Holm–Sidak method.