Systemic first generation (F0) effects of young adult exposure to sevoflurane. The percentage of time spent in open arms of the elevated plus maze, number of crossing the open arms, and distance traveled by male (A–C) and female (D–F) rats. Data are means ± SD from 15 male and 16 female rats per group. (G and H) The percentage of prepulse (PP) inhibition of startle responses at PP intensity of 3, 6, and 12 decibel in male (G) and female (H) rats. Data are means ± SD from 21 males in the Control group, 22 males in the Sevoflurane group, and 16 females per group. *P < 0.05 versus Control. (I–L) The respective levels of serum corticosterone across each collection point, as well as the total corticosterone responses in male (I and J) and female (K and L) rats. Serum levels of corticosterone at rest were taken as baselines for calculations of the total corticosterone responses. Data are means ± SD from 8 rats per group. Color coding of experimental groups in J and L is also applicable to I and K. (M–O) shown are serum levels of testosterone (M), estradiol (N), and testis weight (O) in male rats. (P and Q) Serum levels of testosterone and estradiol in female rats. Data are means ± SD from 8 rats per group. Data of testis weight are from 16 rats in control group and 13 rats in sevoflurane group. *P < 0.05 versus Control.