Molecular first generation (F0) effects of young adult exposure to sevoflurane. (A–F) The levels of hypothalamic gonadotropin-releasing hormone (Gnrh) mRNA, serum levels of luteinizing hormone and follicle-stimulating hormone of male (A–C) and female (D–F) rats. Data are means ± SD from 8 rats per group (n = 6 rats per group in follicle-stimulating hormone). *P < 0.05 versus Control. (G–L) shown are levels of aromatase mRNA, estrogen receptor α gene (Erα) mRNA, and estrogen receptor β gene (Erβ) mRNA in the hypothalamus of male (G–I) and female (J–L) rats. Data normalized against control are means ± SD from 6 rats per group. *P < 0.05 versus Control. (M–P) Shown are the respective levels of hypothalamic corticotropin-releasing hormone gene (Gnrh) mRNA and hippocampal glucocorticoid receptor gene (Gr) mRNA in males (M and N) and females (O and P). Data normalized against control are means ± SD from 6 rats per group (n = 8, male corticotropin-releasing hormone; n = 5 in the Control group and n = 4 in the Sevoflurane group in female glucocorticoid receptor). *P < 0.05 versus Control.