Fig. 2.
c-Fos immunostaining of the spinal dorsal horn in sections from C7-T1 and L4-5 segments of the spinal cord after a moderately cold thermal stimulation of 15°C to paws of mice on day 4 postoxaliplatin (15 mg/kg) or vehicle administration. Ten repetitive stimulation lasting 30 s each were applied to the forepaws and hind paws in 2-min cycles over a total period of 20 min and mice were sacrificed after 2 h. Sections from C7-T1 and L4-5 segments of the spinal cord in an oxaliplatin-administered mouse (A and B, respectively) and section from C7-T1 segment of the spinal cord in vehicle-administered mouse (C). Scale bars are 50 μm.(D) Counts of Fos-like immunoreactive cells in the superficial laminae. The number of Fos-like immunoreactive nuclei in the superficial laminae of the ipsilateral dorsal horn in three random sections per segment was counted and averaged. Data were given as scatter plots of the average number of the Fos-like immunoreactive cells in vehicle- and oxaliplatin-administered mice with mean ± SEM, and analyzed by two-tailed paired t test (n = 4 for each group). NS = not significant; OHP = oxaliplatin; VEH = vehicle.