Effect of cardiomyocyte- and myeloid-specific MyD88 deletion on survival rate and body temperature during endotoxin shock. Both cardiac- (α-MHC-MyD88^−/−) and myeloid- (Lyz-MyD88^−/−) MyD88 deletion mice were subjected to lipopolysaccharide (LPS) (15 mg/kg, intraperitoneal injection) together with the littermates control (MyD88^fl/fl). (A) The diagram of time line of the experiments. The mortality was monitored for up to 96 h (B and C) and body temperature was measured at 6 and 24 h after LPS injection (D and E). Each error bar represents mean ± SD. ** P < 0.01; n = 10 in each group in B and D, n = 15 mice in each group in C and E. Echo. = echocardiography; Lyz-MyD88^−/− = myeloid-specific MyD88 knockout mice; α-MHC = α-myosin heavy chain; α-MHC-MyD88^−/− = cardiomyocyte-specific MyD88 knockout mice; MyD88 = myeloid differentiation factor 88; MyD88^fl/fl = MyD88-loxP control mice; temp. = temperature.