Intracisternal application of the reactive oxygen species donor, tert-butyl hydroperoxide (t-BOOH), induces a static, but not a dynamic, mechanical allodynia. (A, C) Time courses of the changes in behavioral responses (allodynic score) to static (6-g von Frey filament; A) and dynamic mechanical stimuli (air puff; C) applied on the face of rats intracisternally injected with artificial cerebrospinal fluid (aCSF) or increasing doses of t-BOOH: 50, 100, and 250 μg. (B, D) Bar histograms of the corresponding areas under the curves for static (B) and dynamic mechanical stimuli (D). Reactive oxygen species mainly produce a static mechanical allodynia. Data are represented as the mean ± SD, n = 5 per group. *P < 0.05, **P < 0.01, ***P < 0.001.