Fig. 3.
Western blot analysis for succinate dehydrogenase A (SDHA), a protein in electron transport chain complex II. Blots are shown for rat, summer active arctic ground squirrel (AGS Sum or SA-AGS), and hibernating arctic ground squirrel (AGS Win or HIB-AGS) at sham (S), 3 h (R3h), and 24 h of reperfusion (R24h) time points. Heat-shock protein 60 (HSP60) is shown as a mitochondrial protein loading control (A). Densitometric analysis of HSP60 normalized SDHA bands demonstrating down-regulation in SA and HIB-AGS compared to rat under sham, R3h, and R24h conditions, consistent with the mass spectrometry findings (B). Results are displayed as mean ± SEM. Two-way ANOVA analysis identified both species and time point as being significant factors (P < 0.0001) affecting SDHA expression.

Western blot analysis for succinate dehydrogenase A (SDHA), a protein in electron transport chain complex II. Blots are shown for rat, summer active arctic ground squirrel (AGS Sum or SA-AGS), and hibernating arctic ground squirrel (AGS Win or HIB-AGS) at sham (S), 3 h (R3h), and 24 h of reperfusion (R24h) time points. Heat-shock protein 60 (HSP60) is shown as a mitochondrial protein loading control (A). Densitometric analysis of HSP60 normalized SDHA bands demonstrating down-regulation in SA and HIB-AGS compared to rat under sham, R3h, and R24h conditions, consistent with the mass spectrometry findings (B). Results are displayed as mean ± SEM. Two-way ANOVA analysis identified both species and time point as being significant factors (P < 0.0001) affecting SDHA expression.

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