Fig. 1.
Amphiregulin (Areg) expression in lung tissues is up-regulated in lipopolysaccharide-induced acute lung injury. Mice were instilled intratracheally with lipopolysaccharide (3 mg/kg) and killed at indicated time points. (A) Quantitative reverse transcription polymerase chain reaction data for relative gene expression of Areg in the lung tissues. Gene expression was normalized to β-actin expression levels, and the mean value for mice in 0 h group was set to 1. Data are shown as mean ± SD of five independent samples. Concentrations of Areg in lung tissue homogenate (B) and bronchial alveolar lavage fluid (BALF; C) were measured by enzyme-linked immunosorbent assay. Data were shown as mean ± SD from five independent samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus 0 h; one-way ANOVA Bonferroni posttest.