The effects of single intrathecal and intraperitoneal minocycline hydrochloride (MC) administrations on allodynia and hyperalgesia in streptozotocin (STZ)-induced diabetic neuropathic pain in mice at day 7 after STZ administration. The effects of single intrathecal (60 μg/5 μl) or single intraperitoneal (30 mg/kg) MC administration on mechanical allodynia (von Frey test; A) and thermal hyperalgesia (cold plate test; B) were evaluated at 1 and 4 h after administration. Data are presented as the means ± SD (4 to 8 mice per group). The results were evaluated using one-way ANOVA followed by Bonferroni test for comparisons of selected pairs; *P < 0.05 and ***P < 0.001 compared naive mice versus any of the other group; #P < 0.05 and ##P < 0.01 compared the vehicle (V)- versus MC-treated streptozotocin-induced diabetic neuropathic pain in mice; $P < 0.05, $$P < 0.01, and $$$P < 0.001 comparison between 1 and 4 h in MC-treated STZ-induced diabetic neuropathic pain in mice.