Fig. 2.
The effect of twice-daily minocycline hydrochloride (MC; 30 mg/kg, intraperitoneally, for 21 days) treatment on streptozotocin (STZ; 200 mg/kg; intraperitoneally)–induced hyperglycemia, body weight changes, allodynia, and hyperalgesia measured at days 7, 14, and 21 after STZ administration. The effects of single STZ administrations and twice-daily vehicle (V) or MC treatment on plasma glucose (A), body weight (B), allodynia (von Frey test; C), and hyperalgesia (cold plate test; D) are shown. Data are presented as the means ± SD (7 to 30 mice per group). The results were evaluated using one-way ANOVA followed by Bonferroni test for comparisons of selected pairs. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the naive animals; #P < 0.05 and ###P < 0.001 compared the V- versus MC-treated STZ-induced diabetic neuropathic pain in mice at respective time points.