Fig. 4. Effect of perfluorohexane (PFH) on tissue factor (TF) content in cell lysates and on tumor necrosis factor (TNF)α concentrations in supernatants after ex vivo  lipopolysaccharide (LPS) stimulation (10 μg/ml) of human alveolar macrophages prepared from diagnostic bronchoalveolar lavage fluid for 6 h. (A ) Note the increased TF antigen content in lipopolysaccharide-stimulated alveolar macrophages compared with nonstimulated controls, which was significantly inhibited by perfluorohexane ([vol/vol] 30%). Results are presented as mean cellular TF concentration (± SD) from 10 independent experiments; ** P < 0.01. (B ) Note the increased TNFα concentration in supernatants of lipopolysaccharide-stimulated alveolar macrophages compared with nonstimulated controls, which was significantly inhibited by perfluorohexane ([vol/vol] 30%). Results are presented as mean cellular TNFα concentration (± SD) from 10 independent experiments; * P < 0.05.