Fig. 3. (Top ) The effect of substrate concentration on propofol hydroxylase activity in human liver microsomes from representative low-, intermediate-, and high-activity livers. The solid line connects best-fit estimates of these data determined by nonlinear least-squares curve fit to either equation 1(HL9 and HL34) or e quation 2 (HL25). Estimates for K  ^mand V^maxare given in table 1. (Bottom ) Eadie-Hofstee transformation of these data. V = reaction velocity, nmoles · mg⁻¹· min⁻¹; V/[S]= reaction velocity divided by propofol concentration at that velocity, nmoles · mg⁻¹· min⁻¹·μm⁻¹.