Dorsal root ganglion knockdown of PRDM1 attenuates chronic constriction injury–induced hyperalgesia. (A through C) Dorsal root ganglion microinjection of short hairpin RNA attenuates chronic constriction injury–induced mechanical allodynia (A), thermal hyperalgesia (B), and cold hyperalgesia (C). Short hairpin RNA or Scram was injected on day 7 after chronic constriction injury. Data are expressed as mean ± SD. **P < 0.01 and ***P < 0.001 versus the sham + Scram group; #P < 0.05, ##P < 0.01, and ###P < 0.001 versus the chronic constriction injury + Scram group by two-way ANOVA followed by post hoc Bonferroni multiple comparison test, F_{(14, 168)} = 10.10 (A), F_{(14, 168)} = 7.19 (B), and F_{(14, 168)} = 8.08 (C), n = 8 per group. (D and E) Dorsal root ganglion microinjection of short hairpin RNA attenuates chronic constriction injury–induced decrease in paw print area (D) and single stance (E). Data are expressed as mean ± SD. ***P < 0.001 versus the sham + Scram group; #P < 0.05 and ### P < 0.001 versus the chronic constriction injury + Scram group by two-way ANOVA followed by post hoc Bonferroni multiple comparison test, F_{(2, 14)} = 47.12 (D) and F_{(2, 14)} = 14.78 (E), n = 8 per group. (F) Combined paw print image. (G) Representative digitized paw prints and associated step cycles. PRDM1, positive regulatory domain I–binding factor 1.