Fig. 9.
Measurements of transient receptor potential canonical (TRPC) 3 and TRPC6 protein expression in gastrocnemius muscles. (A) A representative Western blot analysis of the expression of TRPC3, TRPC6, and glyceraldehyde-3-phosphate dehydrogenase in the gastrocnemius muscle from wild-type, wild-type/dominant-negative TRPC6, RYR1-p.R163C, and RYR1-p.R163C/dominant-negative TRPC6 mice. (B) Summary of the densitometric analysis. The data were normalized to glyceraldehyde-3-phosphate dehydrogenase and expressed as mean optical unit values ± SD. Three mice per genotype. For TRPC3 and TRPC6 protein expression, the densitometric quantitative data were based on three independent experiments from each mouse. The values are expressed as means ± SD for each condition. Statistical analysis was done using unpaired Student’s independent t test. *P ≤ 0.05.

Measurements of transient receptor potential canonical (TRPC) 3 and TRPC6 protein expression in gastrocnemius muscles. (A) A representative Western blot analysis of the expression of TRPC3, TRPC6, and glyceraldehyde-3-phosphate dehydrogenase in the gastrocnemius muscle from wild-type, wild-type/dominant-negative TRPC6, RYR1-p.R163C, and RYR1-p.R163C/dominant-negative TRPC6 mice. (B) Summary of the densitometric analysis. The data were normalized to glyceraldehyde-3-phosphate dehydrogenase and expressed as mean optical unit values ± SD. Three mice per genotype. For TRPC3 and TRPC6 protein expression, the densitometric quantitative data were based on three independent experiments from each mouse. The values are expressed as means ± SD for each condition. Statistical analysis was done using unpaired Student’s independent t test. *P ≤ 0.05.

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