American Society of Anesthesiologists

Fig. 1.

$Blood preparation and experimental procedure for in vitro study. Whole human blood units were obtained from the French Blood Bank Institute (Rennes, France) after obtaining donor written informed consent. Whole blood unit volume was 478 ml (461 to 511 ml). Citrate–phosphate–dextrose anticoagulated blood units were diluted in normal saline (0.9% NaCl; Macopharma, France) up to 1,200 ml (including 200 ml of collection reservoir priming). Unfractionated heparin (Choay heparin; Sanofi-Aventis, France) at high concentration was added to whole blood before dilution in saline. The final heparin concentration in the collection reservoir was 12 IU/ml. After collection reservoir priming (200 ml of 0.9% NaCl; Macopharma), the blood was collected into the blood collection reservoir under controlled depression level using the suction line, with a vacuum level of −250 mbar. Then the experimental procedure consisted of two consecutive treatment cycles using the i-SEP device. The first cycle was programed to treat 700 ml and the second cycle treated 500 ml (total volume, 1,200 ml).$

Blood preparation and experimental procedure for in vitro study. Whole human blood units were obtained from the French Blood Bank Institute (Rennes, France) after obtaining donor written informed consent. Whole blood unit volume was 478 ml (461 to 511 ml). Citrate–phosphate–dextrose anticoagulated blood units were diluted in normal saline (0.9% NaCl; Macopharma, France) up to 1,200 ml (including 200 ml of collection reservoir priming). Unfractionated heparin (Choay heparin; Sanofi-Aventis, France) at high concentration was added to whole blood before dilution in saline. The final heparin concentration in the collection reservoir was 12 IU/ml. After collection reservoir priming (200 ml of 0.9% NaCl; Macopharma), the blood was collected into the blood collection reservoir under controlled depression level using the suction line, with a vacuum level of −250 mbar. Then the experimental procedure consisted of two consecutive treatment cycles using the i-SEP device. The first cycle was programed to treat 700 ml and the second cycle treated 500 ml (total volume, 1,200 ml).

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