Fig. 5.
(A) Representative traces of single TASK-1 channels expressed in HEK293 cells. Top to bottom: control, halothane 2.5%, isoflurane 2.5% and a mixture of 2.5% halothane and 2.5% isoflurane. In each recording, washout restored the activities back to the level of control. (B) Results of quantitative analysis of TASK-1 channel activation expressed in HEK293 cells (channel open probability [nPopen] values, mean ± SD; n = 81 separate recordings in separate cells). Halothane (red) is a stronger activator than isoflurane (purple) with both agents reaching apparent saturation, and isoflurane (as in fig. 1) appearing to exhibit partial agonist behavior. The red (halothane) and purple (isoflurane) lines are the result of applying Equation 1 to the data, and estimating individual Kd and Bmax values. See Supplementary Digital Content, figure S4 (https://links.lww.com/ALN/C467), for the data plotted against aqueous concentrations.