Fig. 9.
Yes-associated protein (YAP) is involved in atelectasis-associated barrier dysfunction. (A) Representative images for YAP (green) from aerated and atelectatic regions in lipopolysaccharide (–) (n = 4 animals) or lipopolysaccharide (+) (n = 6 animals). Nuclei were stained with Hoechst in blue, and autofluorescent blood cells appear in red; scale bars represent 20 µm. (B) Nuclear YAP intensity was markedly reduced in atelectatic versus aerated regions. Each connected dot pair represents atelectatic and aerated regions from a single animal. (C–E) Real-time polymerase chain reaction validation documenting lower mRNA expression for YAP-responsive genes (C), cytoskeleton-associated proteins (D), and actin dynamics associated factors (E) in atelectatic than in aerated regions (n = 6 animals). P values corresponding to analyzed effects (lipopolysaccharide exposure [Group], lung region [Lung], and their interaction [Group × Lung]) are indicated.