Fig. 10.
Protein kinase A inhibition at the dorsal reticular nucleus produces no effects in mechanical and thermal hypersensitivity induced by chronic morphine. The dorsal reticular nucleus was injected with the protein kinase A inhibitor H-89 or saline 7 days after the implantation of morphine-minipumps. The von Frey (A) and hot-plate (B) tests, which evaluate mechanical and thermal sensitivity, respectively, were performed before and 40 min after injection. The interactions between groups and time are presented at the bottom of the x axis. No significant effects were detected between treatments (saline vs. H-89) both in the von Frey (F1,10 = 2.1, P = 0.175; A) and hot plate (F1,10 = 0.7, P = 0.414; B) tests. Data are presented as mean ± SD (saline n = 6; H-89 n = 6).