Fig. 5.
The effect of neonatal exposure to isoflurane or wild-type PDZ2 peptide on fear learning and memory. At 1 week, mice in cohort 1 were exposed to O2 (control, 100% O2) or isoflurane (1.5% ISO in 98.5% O2) and cohort 2 were injected with PDZ2MUT (8 mg/kg inactive mutant peptide) or PDZ2WT (8 mg/kg active wild-type peptide). Data are plotted showing % freezing duration mean ± SD. (A) Fear conditioning “learning” at 7 weeks: Pre- and postshock comparisons within each group were analyzed with t tests followed by Holm-Sidak multiple comparison correction and all P < 0.0001. (A, left) Control (n =23) versus Isoflurane (n = 30), P < 0.0001; PDZ2MUT (n = 23) versus PDZ2WT (n = 24), P < 0.011. (B) Fear testing “memory” at 8 weeks: Control (n = 23) versus Isoflurane (n = 30), P < 0.0001; PDZ2MUT (n = 23) versus PDZ2WT (n = 24), P < 0.0001. n = number of animals. Data were analyzed with one-way ANOVA followed by Holm-Sidak multiple comparison correction. *P < 0.050, **P < 0.010, ***P < 0.001, ****P < 0.0001.