Difference in brain Tau amount between neonatal and adult mice. (A) Experimental design. Cortex or hippocampus tissues were harvested at the end of the baseline or sevoflurane anesthesia in neonatal (postnatal days 6 to 8) and adult (postnatal days 60 to 62) mice. (B) Difference in brain amounts of Tau and oligomer Tau (T22) in the cortex of postnatal day 6 mice of both sexes (neonatal mice of both sexes) and postnatal day 60 female mice (female reference group mice) in baseline and sevoflurane anesthesia groups. (C) Summary of amounts of Tau (n = 6 mice/group). (D) Summary of amounts of oligomer Tau (T22; n = 6 mice/group). (E) Immunostaining of Tau- and T22-positive cells in the cortex of neonatal mice from of sexes and the female reference group mice in baseline and sevoflurane anesthesia groups. (F) Quantification of Tau-positive cells (n = 3 mice/group). (G) Quantification of T22-positive cells (n = 3 mice/group). (H) Difference in brain amounts of Tau and oligomer Tau (T22) in the cortex of postnatal day 6 mice from of sexes (neonatal mice of both sexes) and postnatal day 60 male mice (male reference group mice) in baseline. (I) Summary of amounts of Tau (n = 6 mice/group). The data are presented as means ± SD. All data are quantified and expressed as arbitrary units or real numbers compared with reference group mice (postnatal day 60) or baseline group (nonanesthetized mice). Two-way ANOVA was used for (C), (D), (F) and (G). Student’s t test was used for (I). ***P < 0.001. DAPI, 4[prime],6[prime]-diamino-2-phenylindole.