Effects of Nuak1 inhibitor HTH-01-015 on Tau and oligomer Tau amounts in neonatal mice of both sexes. (A) Experimental design. The mice were treated with baseline or sevoflurane anesthesia with vehicle (dimethyl sulfoxide [DMSO]) or HTH-01-015. (B) ATP concentration in cortex of neonatal mice of both sexes and female reference mice in baseline and sevoflurane anesthesia groups (n = 6 mice/group). (C) Amounts of Tau and oligomer Tau (T22) in the cortex of neonatal mice of both sexes and female reference group mice. (D) Summary of Tau (n = 6 mice/group). (E) Summary of T22 (n = 6 mice/group). (F) Enzyme-linked immunosorbent assay (ELISA) of total Tau in cortex of neonatal mice of both sexes and female reference group mice (n = 6 mice/group). The data are presented as means ± SD. All data are quantified and expressed as arbitrary units or real number compared with baseline group (nonanesthetized mice). One-way ANOVA was used for (B), (D), (E), and (F). *P < 0.05; ^#P < 0.05.