Calculations of blood flows. (A) Scatter plot for true versus calculated pulmonary blood flow, all individual injections shown. Linear regression yields y = 1.08 * x – 150. Regressions for every animal are found in the Supplemental Digital Content (https://links.lww.com/ALN/C425). (B) Four-quadrant plot showing the differences in calculated blood flow versus the differences in measured blood flow during step 2. Linear regression (forced through origin) yields y = 1.16 * x. The dotted frame represents the zone of exclusion around least significant change. (C) Bland-Altman-Plot for true and calculated pulmonary blood flow, mean of injections after removing outliers. Bias is −6 [95% CI ± 48 ml] with limits of agreement of 668 [95% CI ± 166 ml/min]. Proportional bias is 332 − 0.17 * bias, limits of agreement ± 623 ml, r² = 0.15, P = 0.005. Colors represent different extracorporeal membrane oxygenation blood flows (blue: 4 l/min, red: 3 l/min, yellow: 2 l/min, purple: 1 l/min). Different symbols represent different animals.