Fig. 3.
Intrathecal (IT) injection of liver X receptor (LXR) α/β agonist either before or after spared nerve injury (SNI) attenuated mechanical allodynia induced by SNI. (A, B) T0901317 dose-dependently blocked bilateral mechanical allodynia induced by SNI (n = 5 or 6/group). (C, D) A single IT injection of T0901317 (19.2 µg [C]; n = 7) or GW3965 (6.2 µg [D]; n = 7) 7 days after SNI increased the 50% paw withdrawal threshold in the bilateral side (n = 6/group). (E) A single IT injection of T0901317 into rats that had received SNI surgery 21 days before inhibition of the mechanical allodynia 1 and 2 h after injection. (F) In the sham-operated rats, T0901317 at 19.2 µg had no effect on the paw withdrawal incidence evoked by the four different filaments (n = 5). Arrows show the injection of T0901317 or GW3965. *P < 0.05, **P < 0.01, ***P < 0.001 versus baseline; $$P < 0.01, $$$P < 0.001 versus dimethyl sulfoxide–treated SNI group. DMSO = dimethyl sulfoxide.