Fig. 2.
Expression of CYP2B6.19. CYP2B6.19 was expressed mostly as inactive P420 protein in regular expression medium, suggesting that the expressed protein was unstable. However, when 5% glycerol was added to the cell culture medium ESF AF 24 h postinfection, the active P450 content increased, while P420 content decreased. This approach to improving the expression of unstable CYP2B6 variants has not been previously described.