Fig. 5.
MicroRNA expressions of various microRNAs that target autophagy-related genes and ubiquitin E3 ligases or that promote denervation-induced skeletal muscle atrophy (miR-21-5p and miR-206-3p) in diaphragms of the groups pretreated with saline before undergoing controlled mechanical ventilation for 24 h (MV) and pretreated with N-acetylcysteine before undergoing controlled mechanical ventilation for 24 h (MV-NAC; n = 6 per group), where n refers to the number of animals. The values are means ± SD, expressed as fold change from the group allowed to spontaneously breathe (SB; dotted line). *P < 0.05, compared with SB group. Comparisons between the three experimental groups were performed using one-way ANOVA.