Fig. 8. Western blot analysis illustrating the effect of intrathecal administration of the ς1receptor antagonist BD1047 (30 nmol; n = 4) on days 0–5 or on days 15–20 after surgery on N -methyl-d-aspartate receptor NR1 expression and NR1 phosphorylation (pNR1). The representative blots for vehicle or BD1047 treatment during the induction period (days 0–5) are shown for NR1 in A and for pNR1 in B . The relative pixel area (%) of NR1 and pNR1 expression was dramatically and significantly reduced by BD1047 treatment during the induction period (days 0–5) as compared with those of vehicle-treated chronic constriction injury rats ( C ; n = 4; * P < 0.05), whereas BD1047 treatment during the maintenance period (days 15–20) did not affect NR1 and pNR1 expression in the spinal cord dorsal horn ( D ; n = 4 for each group) .