Figure 1. Effect of endothelin-1 on⁴⁵Ca²+ influx in A10 cells. (A) Time-dependent effect: The cultured cells were treated with 0.1 micro Meter endothelin-1 ([round bullet, filled]) or vehicle ([circle, open]) in the presence of 5 micro Ci⁴⁵Ca²+ for the indicated periods. (B) Dose-dependent effect: The cultured cells were stimulated with various doses of endothelin-1 for 10 min. Values for vehicle-treated cells were subtracted to produce each data point in panel B. Each value represents the mean +/- SD of triplicate determinations of a representative experiment done three times. *P <0.05 versus the value without endothelin-1.