Figure 3. Platelet PAC-1 binding at hypothermic temperature. Platelet-rich plasma was incubated with fluorescein-labeled PAC-1 antibody (4 [micro sign]g/ml) and adenosine diphosphate or thrombin receptor activating peptide for 20 min at the indicated temperatures. Platelet PAC-1 binding was determined using flow cytometric analysis. Results are means +/- SEM. *P < 0.05 versus 37 [degree sign]C, **P < 0.01 versus 37 [degree sign]C. n = 6.