Figure 6. Fluorescence spectra of human serum albumin (hSA) in the absence and presence of F6. (A) Fluorescence emission spectra of hSA equilibrated with the indicated concentrations of F6. (B) The solid line is the difference spectrum obtained by subtracting the fluorescence emission spectrum obtained in the presence of 120 [micro sign]M F6 from the control spectrum (no F6). The dotted line is the control spectrum. Both spectra have been normalized to a peak emission maximum of 1.0 to facilitate comparison.