Fig. 5. Absence of cell death revealed by propidium iodide staining in propofol-treated slice cultures. (A ) Illustration of propidium iodide staining in a healthy control hippocampal slice culture. Only a few labeled cells are present. (B ) Propidium iodide staining obtained after treatment with the neurotoxic agent HgCl2. (C ) Propidium iodide staining of a slice culture treated for 7 days with 100 μg/ml propofol. (D ) Quantitative analysis of the number of propidium iodide–stained cells counted at different times in control (open circles) and propofol-treated (black squares) slice cultures. Data are mean ± SD (n = 6–9).
