Fig. 5. Platelets labeled with fluorochrome carboxyfluorescein succinimidyl ester (CFSE) in the mesenteric venule under Nω-nitro-l-arginine methyl ester (l-NAME; 100 μm) superfusion during 2 MAC sevoflurane anesthesia. (A ) A representative microfluorograph captured at 30 frames/s of the hemin-treated rat. The arrow denotes single platelet labeled with CFSE. (B ) A representative microfluorograph captured at 30 frames/s of the hemin-untreated rat with carbon monoxide superfusion. Note that the rapid movement of CFSE-labeled platelets (arrow) look like a shooting star, indicating the preservation of microcirculatory flows under l-NAME superfusion. (C ) A trans-illumination image of the same venule as (A ). Note that individual erythrocytes packed in venule were visualized, indicating no flow pattern. (D ) A trans-illumination image of the same venule as (B ). Bar = 30 μm.