Fig. 1. Protocol for five randomized groups of guinea pig hearts (n = 12 in each group). After initial stabilization, anesthetic preconditioning (APC) was elicited by exposing hearts to 1.3 mm sevoflurane for 15 min. 5-Hydroxydecanoate (5-HD; 200 μm) was perfused alone or before, during, and after sevoflurane administration for 35 min. Sevoflurane was washed out for 30 min and 5-HD for 15 min. All ischemic hearts then underwent 30 min of global no-flow ischemia and 120 min of reperfusion; nonischemic hearts (CON) served as controls over time.