Fig. 3. Effect of cardiopulmonary bypass (CPB) on GRK2 protein in mononuclear cell cytosol (n = 15). (A ) GRK2 immunoreactivity in mononuclear cell cytosol from patients 7 and 4. The immunoblotting was carried out as described in the methods section. GRK2 lane, 100 ng of purified GRK2; PRE lanes, 30 μg mononuclear cell cytosolic protein from pre-CPB sample; POST lanes, 30 μg mononuclear cell cytosolic protein from post-CPB samples; POD1 lanes, 30 μg mononuclear cell cytosolic protein from POD1 samples. (B ) GRK2 protein expression in POST and POD1 samples from individual patients. Patient numbers are shown to the right. PRE = before surgery; POST = 2 h after completion of CPB; POD1 = 24 h after completion of CPB.

Fig. 3. Effect of cardiopulmonary bypass (CPB) on GRK2 protein in mononuclear cell cytosol (n = 15). (A ) GRK2 immunoreactivity in mononuclear cell cytosol from patients 7 and 4. The immunoblotting was carried out as described in the methods section. GRK2 lane, 100 ng of purified GRK2; PRE lanes, 30 μg mononuclear cell cytosolic protein from pre-CPB sample; POST lanes, 30 μg mononuclear cell cytosolic protein from post-CPB samples; POD1 lanes, 30 μg mononuclear cell cytosolic protein from POD1 samples. (B ) GRK2 protein expression in POST and POD1 samples from individual patients. Patient numbers are shown to the right. PRE = before surgery; POST = 2 h after completion of CPB; POD1 = 24 h after completion of CPB.

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