Fig. 7. Effects of amitriptyline on viability of human polymorphonuclear neutrophils (hPMNs). (A ) Trypan blue staining of hPMNs after 30-min incubation in Hank's balanced salt solution (control group): cells excluded dye (i.e. , were alive). (B ) Trypan blue staining of hPMNs after 30-min incubation in amitriptyline (10−3m): all cells were stained (i.e. , dead) and partially aggregated.