Fig. 2. Effects of intermittent injections of local anesthetic on Ca2+sparks frequency and on spatio-temporal properties of Ca2+sparks in psoas muscle fibers. ( A ) Representative line-scan ΔF/F images acquired on psoas fibers from control and local anesthetic–treated animals with Ca2+sparks frequency in psoas muscle of control and local anesthetic–treated animals. Spatiotemporal analysis of brief (< 100 ms) and localized increases in fluorescence, corresponding to Ca2+sparks, was performed using a biexponential fitting procedure. 12,26 Spark amplitude ( B ), rise time ( C ), decay time ( D ), and full width at half maximum (FWHM; E ) were analyzed and compared between control and local anesthetic–treated animals. Values are mean ± SD (n = 6/group). * P < 0.05 versus control group.