Fig. 1. Effects of isoflurane on the adenosine triphosphate-sensitive potassium (K^ATP) channel activities in native vascular smooth muscle cells. (  A ) Single-channel characteristics of K^ATPchannels in the cell-attached configurations. Membrane potentials were clamped at −60 mV. Zero current levels are indicated by the  horizontal lines marked “0 pA.” Pinacidil (100 μm) and glibenclamide (Glib) (3 μm) were superfused to the bath solution as indicated by the  horizontal solid bars . Effects of isoflurane on the K^ATPchannel activities in the cell-attached (  B ) and inside-out (  C ) configuration. (  D ) The relation between NPo and time for the traces shown in cell-attached (•) and inside-out (○) configuration. Each  vertical bar constitutes measurements from 12 patches (mean ± SD). *  P < 0.05  versus baseline.