Fig. 3. Isoflurane preconditioning improved motor coordination after brain hypoxia–ischemia. ( A ) Speed–latency index of 37-day-old rats with various treatments in the rotarod test. ( B ) Percentage of alternation of 37-day-old rats with various treatments in the Y-maze test. ( C ) Ratio of the investigation times in the second trial over those in the first trial. Results are mean ± SD (n = 9–30). * P < 0.05 compared with control. # P < 0.05 compared with cerebral hypoxia–ischemia only. ∧ P < 0.05 compared with isoflurane preconditioning plus cerebral hypoxia–ischemia. 1400 W = 1 mg/kg N -(3-(aminomethyl)benzyl)acetamidine injected intraperitoneally 30 min before the isoflurane exposure or 24 h before the cerebral hypoxia–ischemia; HI = cerebral hypoxia–ischemia that was induced by left common carotid artery ligation plus hypoxia with 8% O2for 2 h at 37°C to 7-day-old rats; Iso = 1.5% isoflurane for 30 min.