Fig. 4. UNC-13 is required for isoflurane sensitivity. (  A ) Proteins known to bind to syntaxin. The proteins are aligned with the syntaxin region, with which it binds. Ca²⁺Ch-N- and P-type calcium channels,  28,54,mUNC-18-mammalian UNC-18,  55,56,mUNC-13-mammalian UNC-13,  27,Gβ-β subunit of a G protein,  28,complexin,  57,58,synaptotagmin,  55,tomosyn,  59,SNAP-25,  60,vesicle-associated membrane protein (VAMP).  60,61 (  B ) Isoflurane sensitivity of the locomotion of strains with loss-of-function mutations in  unc-13 and  unc-2 . The full genotypes are  tom-1(ok285 null  ) unc-13(s69 lf  );unc-64(js115 null  );oxIs34 [  unc-64 (L166A/E167A);P  myo-2 ::GFP],  tom-1(ok285 null  ) unc-13(e376 lf  );unc-64(js115 null  );oxIs34 [  unc-64 (L166A/E167A);P  myo-2 ::GFP]/+, and  unc-2(e55 null  ) . Each point represents the mean ± SEM of 10 animals. (  C ) Summary of isoflurane EC⁵⁰s ± SE of the fit from  B .  tom-1(ok285);unc-64(js115);oxIs34 is the genetic background for the  unc-13 mutants and is shown for comparison along with the wild-type strain N2. *  P < 0.01  versus N2 and  ok285;js115;oxIs34 .